Protein Expression Vectors - Design Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]

Protein Expression Vectors - Design Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]

Protein Expression Vectors - Template, Codon Bias, Affinity Tags and Epitopes [Part 2]

Protein Expression Vectors - Template, Codon Bias, Affinity Tags and Epitopes [Part 2]

Protein Expression Vectors - Expression Host and Recombinant Proteins [Part 1]

Protein Expression Vectors - Expression Host and Recombinant Proteins [Part 1]

RNA Expression Vectors - Design, Applications, and Examples (In vitro Transcription)

RNA Expression Vectors - Design, Applications, and Examples (In vitro Transcription)

Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning

Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning

Gateway Cloning: Simple and Multisite Gateway Cloning - LR and BP Cloning

Gateway Cloning: Simple and Multisite Gateway Cloning - LR and BP Cloning

TOPO Cloning - TOPO-Blunt, TOPO-TA, TOPO-directional

TOPO Cloning - TOPO-Blunt, TOPO-TA, TOPO-directional

Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning

Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning

Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer

Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer

Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors

Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors

P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality

P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality

F plasmid, BACs, Fosmid cloning vector - Fosmids are small BACs (Bacterial Artificial Chromosomes)

F plasmid, BACs, Fosmid cloning vector - Fosmids are small BACs (Bacterial Artificial Chromosomes)

Lambda Phage and Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus

Lambda Phage and Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus

Plasmid and Phagemid cloning vector - Selectable vs. Screening marker

Plasmid and Phagemid cloning vector - Selectable vs. Screening marker

Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes

Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes

Restriction Enzymes in Molecular Cloning

Restriction Enzymes in Molecular Cloning

Genetic Engineering - Classic vs Modern methods

Genetic Engineering - Classic vs Modern methods

RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps

RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps

Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)

Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)

Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 + PhiX174)

Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 + PhiX174)

Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)

Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)

Telomere DNA Replication - Chromosome end replication problem in eukaryotes

Telomere DNA Replication - Chromosome end replication problem in eukaryotes

DNA Replication in Space and Time

DNA Replication in Space and Time

Epigenetic Replication during DNA Replication - Replicating histones and DNA methylation

Epigenetic Replication during DNA Replication - Replicating histones and DNA methylation

DNA Replication (Eukaryote) TERMINATION - Non-specific vs Site-specific Termination Mechanism

DNA Replication (Eukaryote) TERMINATION - Non-specific vs Site-specific Termination Mechanism

DNA Replication (Eukaryote) ELONGATION - Polymerase Switching + different types of DNA polymerase

DNA Replication (Eukaryote) ELONGATION - Polymerase Switching + different types of DNA polymerase

DNA Replication (Eukaryote) INITIATION - Origin Firing - CMG Helicase forms the Replisome

DNA Replication (Eukaryote) INITIATION - Origin Firing - CMG Helicase forms the Replisome

DNA Replication (Eukaryote) INITIATION - Origin Licensing - cdt1 recruits Helicase Double Hexamer

DNA Replication (Eukaryote) INITIATION - Origin Licensing - cdt1 recruits Helicase Double Hexamer

DNA Replication (Eukaryote) INITIATION - Origin Recognition by ORCs and cdc6

DNA Replication (Eukaryote) INITIATION - Origin Recognition by ORCs and cdc6

DNA Replication (Prokaryote) TERMINATION - Ter sites and Tus protein together stop DNA replication

DNA Replication (Prokaryote) TERMINATION - Ter sites and Tus protein together stop DNA replication