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Protein Expression Vectors - Design Examples (Fusion Proteins, Insulin, and Cas9) [Part 3]
6 days ago
13:10
Protein Expression Vectors - Template, Codon Bias, Affinity Tags and Epitopes [Part 2]
13 days ago
15:33
Protein Expression Vectors - Expression Host and Recombinant Proteins [Part 1]
2 weeks ago
10:00
RNA Expression Vectors - Design, Applications, and Examples (In vitro Transcription)
3 weeks ago
13:18
Golden Gate Cloning or assembly - Type IIS (BsaI) restriction enzyme based cloning
3 months ago
7:40
Gateway Cloning: Simple and Multisite Gateway Cloning - LR and BP Cloning
5 months ago
14:42
TOPO Cloning - TOPO-Blunt, TOPO-TA, TOPO-directional
8 months ago
15:08
Cloning with Restriction Enzymes (5 Levels) - Traditional Molecular Cloning
9 months ago
16:58
Plant cloning vectors - Disarmed Ti plasmid, Cointegrate vectors, Binary vectors - T DNA transfer
9 months ago
14:12
Yeast cloning vectors - YIp, YEp, YRp, YCp, YACs - Shuttle plasmid vectors
9 months ago
21:45
P1 Phage and PAC cloning vector - Pac sites, Cre-loxP, and SacB lethality
10 months ago
16:06
F plasmid, BACs, Fosmid cloning vector - Fosmids are small BACs (Bacterial Artificial Chromosomes)
10 months ago
11:51
Lambda Phage and Cosmid cloning vector - spi phenotype and in vitro packaged lambda virus
10 months ago
15:01
Plasmid and Phagemid cloning vector - Selectable vs. Screening marker
10 months ago
26:22
Dam and Dcm methylation (restriction independent methyl-transferases) affects restriction enzymes
11 months ago
10:35
Restriction Enzymes in Molecular Cloning
11 months ago
26:59
Genetic Engineering - Classic vs Modern methods
11 months ago
6:27
RNA Polymerase III Transcription - Promoters, TFs, Initiation, Elongation, and Termination steps
1 year ago
9:56
Prokaryotic Transcription Factors in Elongation and Termination (GreA, GreB, Mfd, NusA)
1 year ago
8:38
Rolling Circle DNA Replication and Amplification - Plasmids and Bacteriophages (M13 + PhiX174)
1 year ago
15:18
Mitochondrial DNA (mtDNA) Replication - D loop Replication (deep-dive video)
1 year ago
17:41
Telomere DNA Replication - Chromosome end replication problem in eukaryotes
1 year ago
15:23
DNA Replication in Space and Time
1 year ago
12:06
Epigenetic Replication during DNA Replication - Replicating histones and DNA methylation
1 year ago
18:30
DNA Replication (Eukaryote) TERMINATION - Non-specific vs Site-specific Termination Mechanism
1 year ago
10:03
DNA Replication (Eukaryote) ELONGATION - Polymerase Switching + different types of DNA polymerase
1 year ago
11:42
DNA Replication (Eukaryote) INITIATION - Origin Firing - CMG Helicase forms the Replisome
1 year ago
12:35
DNA Replication (Eukaryote) INITIATION - Origin Licensing - cdt1 recruits Helicase Double Hexamer
1 year ago
10:01
DNA Replication (Eukaryote) INITIATION - Origin Recognition by ORCs and cdc6
1 year ago
13:19
DNA Replication (Prokaryote) TERMINATION - Ter sites and Tus protein together stop DNA replication
1 year ago
17:59